Effects of Radiographic Contrast Media on the Micromorphology of the Junctional Complex of Erythrocytes Visualized by Immunocytology

نویسندگان

  • Ralf-Peter Franke
  • Anne Krüger
  • Tim Scharnweber
  • Folker Wenzel
  • Friedrich Jung
چکیده

Effects of radiographic contrast media (RCM) application were demonstrated in vitro and in vivo where the injection of RCM into the A. axillaris of patients with coronary artery disease was followed by a significant and RCM-dependent decrease of erythrocyte velocity in downstream skin capillaries. Another study in pigs revealed that the deceleration of erythrocytes coincided with a significant reduction of the oxygen partial pressure in the myocardium--supplied by the left coronary artery--after the administration of RCM into this artery. Further reports showed RCM dependent alterations of erythrocytes like echinocyte formation and exocytosis, sequestration of actin or band 3 and the buckling of endothelial cells coinciding with a formation of interendothelial fenestrations leading to areas devoid of endothelial cells. Key to morphological alterations of erythrocytes is the membrane cytoskeleton, which is linked to the band 3 in the erythrocyte membrane via the junctional complex. Fundamental observations regarding the cell biological and biochemical aspects of the structure and function of the cell membrane and the membrane cytoskeleton of erythrocytes have been reported. This review focuses on recent results gained, e.g., by advanced confocal laser scanning microscopy of different double-stained structural elements of the erythrocyte membrane cytoskeleton.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Proximal junctional kyphosis in adolescent idiopathic scoliosis following segmental posterior spinal instrumentation and fusion minimum 2 years follow-up

  Abstract   Background: To evaluate proximal junctional segment changes in Adolescent Idiopathic   Scoliosis(AIS) the posterior spinal fusion and also instrumentation also and   finding of probable risk factors, were all considered in this study.   Methods: We retrospectively reviewed radiographs of 121 consecutive patients   who underwent posterior spinal fusion for AIS from T3 or below, with...

متن کامل

Assessment of Contrast Media Volume Injected and Image Contrast in Abdominal Pelvic CT Scan and Related Factors in Referral Hospitals in Mazandaran, Iran

 Background and purpose: Computed tomography scan (CT) is highly capable of diagnosing abdominal and pelvic lesions. Injection of contrast media at appropriate concentration and volume provides the least side effects and the highest image quality. This study aimed at investigating the volume of contrast medium injected and the level of image contrast compared to the standard model, in the imagi...

متن کامل

Effect of a static magnetic field on bone healing in the dog: radiographic and histopathological studies

Although the promotional effects on bone healing of pulsed electromagnetic fields (PEMF) have beenwell demonstrated, the effects of static magnetic fields (SMF) remained unclear. In this study, effects of acustom-made magnetic wrap on radiographic and histopathological aspects of bone healing using a canineunstable osteotomy gap model were investigated. After an osteotomy of the midshaft radius...

متن کامل

Differentiation of Glomerular from Non-Glomerular Hematuria by Three Different Me-thods of Microscopic Examinations of Erythrocytes in Urine

Background: Morphological examinations of urinary erythrocytes can be of diagnostic value in initial evaluation of hematuria. Dysmorphic urinary red blood cells are known to indicate a glomerular origin of bleeding. We examined the clinical usefulness of this test in a population complained of hematuria by use of three different methods: light microscopy, phase contrast microscopy, and Wright s...

متن کامل

Combined Effect of Iodine Contrast Media, Cisplatin and External Beam Radiotherapy on Anaplastic Thyroid Cancer Cells

Introduction: The current study investigated the combination of high Z atoms (iodine-, platinium-based drugs) with using low energy irradiation (120kvp) in Anaplastic Thyroid cancer cells.Materials and Methods: For this purpose, eight groups were designed: control (CNT), different concentrations of Iodine contrast media (ICM),  irradiation with various doses, Cis-platin (CDDP) with different c...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 15  شماره 

صفحات  -

تاریخ انتشار 2014